Authors
Andrés F Parguiña, Lilian Grigorian-Shamagian, Rosa M Agra, Diego López-Otero, Isaac Rosa, Jana Alonso, Elvis Teijeira-Fernández, José Ramón González-Juanatey, Ángel García
Publication date
2011/12
Journal
Arteriosclerosis, thrombosis, and vascular biology
Volume
31
Issue
12
Pages
2957-2964
Publisher
Lippincott Williams & Wilkins
Description
Objective
Our aim in this study was to provide novel information on the molecular mechanisms playing a major role in the unwanted platelet activation associated with ST-elevation myocardial infarction (STEMI).
Methods and Results
We compared the platelet proteome of 11 STEMI patients to a matched control group of 15 stable chronic ischemic cardiopathy patients. In addition, we did a prospective study to follow the STEMI patients over time. Proteins were separated by high-resolution 2D gel electrophoresis, identified by mass spectrometry, and validated by Western blotting. Platelets from STEMI patients on admission displayed 56 protein spot differences (corresponding to 42 unique genes) compared with the control group. The number of differences decreased with time during the patients' follow-up. Interestingly, the adapter protein CrkL and the active form of Src (phosphorylated in Tyr418) were found to be …
Scholar articles
AF Parguiña, L Grigorian-Shamagian, RM Agra… - Arteriosclerosis, thrombosis, and vascular biology, 2011