Authors
Paula Vélez, Raymundo Ocaranza‐Sánchez, Diego López‐Otero, Lilian Grigorian‐Shamagian, Isaac Rosa, Susana Belén Bravo, José Ramón González‐Juanatey, Ángel García
Publication date
2016/8
Journal
PROTEOMICS–Clinical Applications
Volume
10
Issue
8
Pages
851-858
Description
Purpose
Platelets play a fundamental role in the atherothrombotic events that lead to an acute myocardial infarction. In the present study we compared the proteome of intracoronary and peripheral arterial platelets from ST‐elevation myocardial infarction (STEMI) patients in the search for potential platelet biomarkers/drug targets related to what is happening at the culprit site.
Experimental design
Ten STEMI patients were recruited and blood collected from the occluded coronary artery, at the culprit site, in the moment of reperfusion. Systemic blood obtained from the radial artery of the same patients was used as control. Proteome analysis was based on high‐resolution 2D‐DIGE and mass spectrometry. Validations were by western blotting in a group of 11 patients.
Results
Sixteen differentially regulated protein features were identified, corresponding to 15 ORFs, mostly related to cytoskeletal and signaling proteins …
Scholar articles
P Vélez, R Ocaranza‐Sánchez, D López‐Otero… - PROTEOMICS–Clinical Applications, 2016