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This study was conducted to obtain a database describing the nutritional value of freshwater mixed zooplankton that are widely used for larval and grow-out rearing of freshwater fish. The macro and micronutrient composition of mixed zooplankton samples collected from 6 fertilized earthen ponds were analysed for protein, lipid, carbohydrate, ash and these ranged from 73–79%, 10.79–14.55%, 3–4.79% and 3.20–10.07%, respectively on a dry matter (DM) basis. Amino acid profile showed the presence of all the ten essential amino acids with low level of methionine. The content of saturated fatty acids (SAFA), mono unsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) ranged from 64–81%, 7–15% and 6–20% of total fatty acids, respectively. The predominant fatty acids were SAFA (16:0), MUFA (18:1n-9), PUFA viz. linoleic acid (LA 18:2 n-6) and linolenic acid (LNA 18:3 n-3). Among the vitamins …