Authors
Trissa Miller, Nevan J Krogan, Jim Dover, H Erdjument-Bromage, Paul Tempst, Mark Johnston, Jack F Greenblatt, Ali Shilatifard
Publication date
2001/11/6
Journal
Proceedings of the National Academy of Sciences
Volume
98
Issue
23
Pages
12902-12907
Publisher
The National Academy of Sciences
Description
The trithorax genes encode an evolutionarily conserved family of proteins that function to maintain specific patterns of gene expression throughout cellular development. Members of this protein family contain a highly conserved 130- to 140-amino acid motif termed the SET domain. We report the purification and molecular identification of the subunits of a protein complex in the yeast Saccharomyces cerevisiae that includes the trithorax-related protein Set1. This protein complex, which we have named COMPASS (Complex Proteins Associated with Set1), consists of seven polypeptides ranging from 130 to 25 kDa. The same seven proteins were identified in COMPASS purified either by conventional biochemical chromatography or tandem-affinity tagging of the individual subunits of the complex. Null mutants missing any one of the six nonessential subunits of COMPASS grow more slowly than wild-type cells under …
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T Miller, NJ Krogan, J Dover, H Erdjument-Bromage… - Proceedings of the National Academy of Sciences, 2001
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