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The effects of variation in moisture content on the moulding properties of River Niger sand (Onitsha deposit) bonded with Ukpor clay has been investigated in this research. The samples were prepared by washing and sorting to remove impurities and other foreign objects. Weighed samples were sieved and mixed in a Ridsdale laboratory sand mixer (Serial No: 845). American Foundrymen’s Society (AFS) standard test specimens (50mm diameter by 50mm height) were prepared using Ridsdale laboratory sand rammer and moulding properties such as green compression strength, dry compression strength, green shear strength, dry shear strength, green permeability and mouldability were measured using universal sand strength testing machine (Serial No: M8415), electric permeability meter (Serial No: 872) and mouldability tester. Results obtained showed variations in green and dry strengths (Compression and shear), green permeability and mouldability with moisture content. Moisture contents of 4%, 5% and 6% produced maximum values for green compression and green shear strengths (43.40 KN/m2 and 24.50 KN/m2), dry compression and dry shear strengths (395.50 KN/m2and 437.50 KN/m2) and green permeability number (12.00) respectively. Percentage mouldability was highest (98.80%) at 3% moisture content and very low above 5% moisture content. The results of moulding sand tests obtained from the produced synthetic moulding sand indicated that the sand could be used in the production of cast iron and non-ferrous alloys castings.