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We surveyed the lowland rainforest of the Forêt d’Ambre Special Reserve in north Madagascar for amphibians and reptiles. We recorded a total of 20 amphibian and 39 reptile species via opportunistic searching and pitfall trapping in the first published survey to focus on this area. Consequently most of the species found were new records for the area. Approximately half of the species (51%) were only found in relatively undisturbed areas of forest and 61% appear to be restricted to lowland rainforest below 900 m elevation. The most vulnerable elements of this herpetofauna are the three species that appear to be locally endemic to Forêt d’Ambre (according to the current knowledge):