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MicroRNAs (miRNAs) regulate the function of their target genes by down-regulating gene expression, participating in various biological processes. Since the discovery of the first miRNA, computational tools have been essential to predict targets of given miRNAs that can be biologically verified. The precise mechanism underlying miRNA–mRNA interaction has not yet been elucidated completely, and it is still difficult to predict miRNA targets computationally in a robust fashion, despite the large number of in silico prediction methodologies in existence. Because of this limitation, different target prediction tools often report different and (occasionally conflicting) sets of targets. Therefore, we propose a novel target prediction methodology called stacking-based miRNA interaction learner ensemble (SMILE) that employs the concept of stacked generalization (stacking), which is a type of ensemble learning that integrates …