Authors
Kerry O’Donnell, Cécile Gueidan, Stacy Sink, Peter R Johnston, Pedro W Crous, Anthony Glenn, Ron Riley, Nicholas C Zitomer, Patrick Colyer, Cees Waalwijk, Theo van der Lee, Antonio Moretti, Seogchan Kang, Hye-Seon Kim, David M Geiser, Jean H Juba, Robert P Baayen, Matthew G Cromey, Sean Bithell, Deanna A Sutton, Kerstin Skovgaard, Randy Ploetz, H Corby Kistler, Monica Elliott, Mike Davis, Brice AJ Sarver
Publication date
2009/12/1
Journal
Fungal Genetics and Biology
Volume
46
Issue
12
Pages
936-948
Publisher
Academic Press
Description
We constructed a two-locus database, comprising partial translation elongation factor (EF-1α) gene sequences and nearly full-length sequences of the nuclear ribosomal intergenic spacer region (IGS rDNA) for 850 isolates spanning the phylogenetic breadth of the Fusarium oxysporum species complex (FOSC). Of the 850 isolates typed, 101 EF-1α, 203 IGS rDNA, and 256 two-locus sequence types (STs) were differentiated. Analysis of the combined dataset suggests that two-thirds of the STs might be associated with a single host plant. This analysis also revealed that the 26 STs associated with human mycoses were genetically diverse, including several which appear to be nosocomial in origin. A congruence analysis, comparing partial EF-1α and IGS rDNA bootstrap consensus, identified a significant number of conflicting relationships dispersed throughout the bipartitions, suggesting that some of the IGS rDNA …
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