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METHODS

After animal care committee approval, chronic cystitis was induced in three groups of C57/BL6 mice using multiple weight-based intraperitoneal cyclophosphamide (CYP) injections over a period of 3 months. Retrieved bladders at multiple time points were analyzed using histology and immunohistochemistry to assess the severity of inflammation in comparison to untreated control mice. MethyLight assay was performed on bladder inflammation specimens to monitor epigenetic (DNA methylation) changes in comparison to control specimens.

RESULTS

H & E staining showed extensive edema and urothelial sloughing at 1 month of CYP injections, moderate edema at the 2-month time point, and resolution of the edema at the 3-month time point. PCNA staining revealed increased cell proliferation in the epithelial layer at the 1-month time point, but not at the 2-or 3-month time points. MethyLight assay …