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Mobile learning (m-learning) is a relatively new learning tool in the pedagogical arsenal to support students and teachers as they navigate the options available in the expanding world of distance learning. Regarding to wide application of mobile learning, investigating learners’ acceptance toward it is an essential issue. This research investigates learners’ acceptance toward m-learning. An m-learning questionnaire survey that based on 168 university students’ attitudes was investigated. From statistical results, learner autonomy of using m-learning, perceived interaction of using m-learning, quality of m-learning functions, and perceived satisfaction of using m-learning are positive predictors on m-learning acceptance.