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This study includes 10 samples isolated from a different source. This research paper was to study the antibacterial activity and antibiofilm activity of an alcoholic extract of Lemon Myrtle (Backhousia citriodora) leaves, against 10 clinical isolates of Pseudomonas aerogenoza. Isolates were diagnosed by the VITEK-2 compact system and (PCR). The capacity of isolates to produce biofilm was tested by using the Microtiter plate technique (96 wells). The results showed that isolates were able to form a biofilm, also the ability of Backhousia citriodora was been effective on bacteria and biofilm formation.