Authors
Jason T Ladner, Sierra N Henson, Annalee S Boyle, Anna L Engelbrektson, Zane W Fink, Fatima Rahee, Jonathan D’ambrozio, Kurt E Schaecher, Mars Stone, Wenjuan Dong, Sanjeet Dadwal, Jianhua Yu, Michael A Caligiuri, Piotr Cieplak, Magnar Bjørås, Mona H Fenstad, Svein A Nordbø, Denis E Kainov, Norihito Muranaka, Mark S Chee, Sergey A Shiryaev, John A Altin
Publication date
2021/1/19
Journal
Cell Reports Medicine
Volume
2
Issue
1
Publisher
Elsevier
Description
The SARS-CoV-2 proteome shares regions of conservation with endemic human coronaviruses (CoVs), but it remains unknown to what extent these may be cross-recognized by the antibody response. Here, we study cross-reactivity using a highly multiplexed peptide assay (PepSeq) to generate an epitope-resolved view of IgG reactivity across all human CoVs in both COVID-19 convalescent and negative donors. PepSeq resolves epitopes across the SARS-CoV-2 Spike and Nucleocapsid proteins that are commonly targeted in convalescent donors, including several sites also recognized in some uninfected controls. By comparing patterns of homologous reactivity between CoVs and using targeted antibody-depletion experiments, we demonstrate that SARS-CoV-2 elicits antibodies that cross-recognize pandemic and endemic CoV antigens at two Spike S2 subunit epitopes. We further show that these cross …
Scholar articles
JT Ladner, SN Henson, AS Boyle, AL Engelbrektson… - Cell Reports Medicine, 2021