Authors
Wen-Yang Tsai, Han Ha Youn, Carlos Brites, Jih-Jin Tsai, Jasmine Tyson, Celia Pedroso, Jan Felix Drexler, Mars Stone, Graham Simmons, Michael P Busch, Marion Lanteri, Susan L Stramer, Angel Balmaseda, Eva Harris, Wei-Kung Wang
Publication date
2017/11/13
Journal
Clinical Infectious Diseases
Volume
65
Issue
11
Pages
1829-1836
Publisher
Oxford University Press
Description
Background
The explosive spread of Zika virus (ZIKV) and associated microcephaly present an urgent need for sensitive and specific serodiagnostic tests, particularly for pregnant women in dengue virus (DENV)–endemic regions. Recent reports of enhanced ZIKV replication by dengue-immune sera have raised concerns about the role of previous DENV infection on the risk and severity of microcephaly and other ZIKV complications.
Methods
Enzyme-linked immunosorbent assays (ELISAs) based on ZIKV and DENV nonstructural protein 1 (NS1) were established to test acute, convalescent phase, and post–convalescent phase serum/plasma samples from reverse-transcription polymerase chain reaction–confirmed cases including 20 primary ZIKV, 25 ZIKV with previous DENV, 58 secondary DENV, and 16 primary DENV1 infections.
Results
ZIKV-NS1 …
Scholar articles
WY Tsai, HH Youn, C Brites, JJ Tsai, J Tyson… - Clinical Infectious Diseases, 2017