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Dengue virus causes acute febrile illness in tropical and sub-tropical settings and its clinical manifestations ranges from mild form of dengue fever (DF) to the more severe forms of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). A range of diagnostic methods has been developed to support patient management and disease control. The aim of the study is to determine the prevalence of Dengue Fever and Dengue Hemorrhagic Fever in cases presenting with Clinical features suggestive of suggestive of Dengue in a tertiary hospital. The present study was conducted in the Department of Microbiology, SV Medical College, Tirupati from August 2007 to July 2008. Results: Seasonal trend shows the incidences of more number of cases along with positivity were seen in the month of August and October.