Authors
Archana Vijay Gaikwad, Wenying Lu, Surajit Dey, Prem Bhattarai, Greg Haug, Josie Larby, Collin Chia, Jade Jaffar, Glen Westall, Gurpreet Kaur Singhera, Tillie-Louise Hackett, Mathew Suji Eapen, Sukhwinder Singh Sohal
Publication date
2023/3/1
Journal
ERJ open research
Volume
9
Issue
2
Publisher
European Respiratory Society
Description
Background
We have previously reported arterial remodelling in patients with idiopathic pulmonary fibrosis (IPF) and suggested that endothelial-to-mesenchymal transition (EndMT) might be central to these changes. This study aims to provide evidence for active EndMT in IPF patients.
Methods
Lung resections from 13 patients with IPF and 15 normal controls (NCs) were immunostained for EndMT biomarkers: vascular endothelial cadherin (VE-cadherin), neural cadherin (N-cadherin), S100A4 and vimentin. Pulmonary arteries were analysed for EndMT markers by using computer- and microscope-assisted image analysis software Image ProPlus7.0. All the analysis was done with observer blinded to subject and diagnosis.
Results
Increased expression of mesenchymal markers N-cadherin (p<0.0001), vimentin (p<0.0001) and S100A4 (p<0.05) was noted with downregulation of junctional endothelial VE-cadherin (p<0 …
Total citations
Scholar articles
AV Gaikwad, W Lu, S Dey, P Bhattarai, G Haug, J Larby… - ERJ open research, 2023