Authors
Archana Vijay Gaikwad, Wenying Lu, Surajit Dey, Prem Bhattarai, Collin Chia, Josie Larby, Greg Haug, Stephen Myers, Jade Jaffar, Glen Westall, Gurpreet Kaur Singhera, Tillie-Louise Hackett, James Markos, Mathew Suji Eapen, Sukhwinder Singh Sohal
Publication date
2022/1/1
Journal
ERJ open research
Publisher
European Respiratory Society
Description
Background
IPF is a progressive, irreversible fibrotic interstitial lung disease. We performed size-based quantitation of pulmonary arterial remodelling in IPF, examined the role of EndMT and effects on lung physiology.
Methods
Resected lung tissues from 11 normal controls (NC), and 13 IPF patients, were differentially stained using the Movat Pentachrome technique. Size-based classification for pulmonary arteries was conducted in NC and IPF tissues. For each pulmonary artery, arterial size, luminal diameter, thickness of the intima, media, adventitia, and elastin deposition was quantified using Image ProPlus7.0 software. In addition, immunohistochemical staining was performed for EndMT markers and collagen.
Results
Large and medium size arterial numbers were significantly reduced in IPF compared to NC (p<0.0001). Intima thickness was highest in the arterial range of 200–399 μm and 600–1000 μm (p<0 …
Total citations
Scholar articles
AV Gaikwad, W Lu, S Dey, P Bhattarai, C Chia, J Larby… - ERJ open research, 2022