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Four fungal isolates: Simplicillium chinense (iso 9, accession no. KX425621), Penicillium simplicissimum (iso 10, KP713758), Trichoderma asperellum (iso 11, KP792512), and Coriolopsis sp. (1c3, KM403574) were subjected to a series of induced-tolerance training under high metal concentrations to determine if greater tolerance could be achieved from constant exposure to such conditions. Adaptive tolerance assay (Tolerance Index, TI) and Field-Emission Scanning Electron Microscopy with Energy Dispersive X-ray (SEM-EDX) characterized their metal tolerance. “Untrained” S. chinense, P. simplicissimum and T. asperellum showed tolerance towards 4000–4500 ppm Al(III) (TI: 0.64–0.71), 1000 ppm Cr(III) (0.52–0.83) and Pb(II) (0.32–0.88). With tolerance training, tolerance towards 2000–6000 ppm Al(III), 500–3000 ppm Pb(II) and 2000–3000 ppm Cr(III) were achieved (TI: 0.01–0.82) compared to untrained …