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A Gold–Platinum nanoparticle-decorated titania nanotubular electrode is fabricated by electrochemically depositing Au and Pt nanoparticles onto a highly-oriented titania nanotube array. The prepared electrode, characterized by SEM and EDX, shows remarkably improved catalytic activities in the oxidation of hydrogen peroxide. By modifying the electrode with glucose oxidase (GOx) the resultant glucose biosensor exhibits a high sensitivity to glucose in the range of 0 to 1.8 mM with a response time of 3 s and detection limit of 0.1 mM.