Authors
Rupert Kaul, Francis A Plummer, Joshua Kimani, Tao Dong, Peter Kiama, Timothy Rostron, Ephantus Njagi, Kelly S MacDonald, Job J Bwayo, Andrew J McMichael, Sarah L Rowland-Jones
Publication date
2000/2/1
Journal
The Journal of Immunology
Volume
164
Issue
3
Pages
1602-1611
Publisher
American Association of Immunologists
Description
Understanding how individuals with a high degree of HIV exposure avoid persistent infection is paramount to HIV vaccine design. Evidence suggests that mucosal immunity, particularly virus-specific CTL, could be critically important in protection against sexually acquired HIV infection. Therefore, we have looked for the presence of HIV-specific CD8+ T cells in cervical mononuclear cells from a subgroup of highly HIV-exposed but persistently seronegative female sex workers in Nairobi. An enzyme-linked immunospot assay was used to measure IFN-γ release in response to known class I HLA-restricted CTL epitope peptides using effector cells from the blood and cervix of HIV-1-resistant and-infected sex workers and from lower-risk uninfected controls. Eleven of 16 resistant sex workers had HIV-specific CD8+ T cells in the cervix, and a similar number had detectable responses in blood. Where both blood and …
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R Kaul, FA Plummer, J Kimani, T Dong, P Kiama… - The Journal of Immunology, 2000