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Bergenia ciliata of Nepalese origin has been investigated for pharmacognostical, phytochemical and biological activities. The plant was subjected for extraction by ethanol, distilled water, and butanol: hexane (2:1) successfully. The preliminary phytochemical studies of the plant showed the presence of alkaloids, carbohydrates, cardiac glycosides, saponins, phenols, flavonoids, diterpenes and absence of phytosterols. Antimicrobial activity of plant extracts was carried out by cup diffusion method using Staphylococcus aureus, Streptococcus faecalis, Escherichia coli and Pseudomonas aeruginosa bacteria. The root and leaf extract showed activity against tested microorganisms. Brine shrimp bioassay revealed that ethanol extracts of the rhizome of plant had cytotoxic property against brine shrimp nauplii.