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The terrestrial biosphere can release or absorb the greenhouse gases, carbon dioxide (CO₂), methane (CH₄) and nitrous oxide (N₂O), and therefore has an important role in regulating atmospheric composition and climate. Anthropogenic activities such as land-use change, agriculture and waste management have altered terrestrial biogenic greenhouse gas fluxes, and the resulting increases in methane and nitrous oxide emissions in particular can contribute to climate change^,. The terrestrial biogenic fluxes of individual greenhouse gases have been studied extensively^,,, but the net biogenic greenhouse gas balance resulting from anthropogenic activities and its effect on the climate system remains uncertain. Here we use bottom-up (inventory, statistical extrapolation of local flux measurements, and process-based modelling) and top-down (atmospheric inversions) approaches to quantify the global net biogenic …