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Choosing the effective disinfectants and using the standard methods of sterilization in hospitals can be useful in decreasing NOSOCOMIAL INFECTIONS. The aims of this study were the evaluation of efficacy of disinfectants being used in SURGICAL ROOM s, ICU, CCU, burn wards and delivery rooms of Hamedan hospitals and also determination of BACTERIAL CONTAMINATION. In this study 400 samples were collected from different parts of operation rooms, ICU, CCU and delivery rooms of four hospitals (Mobasher Kashani, Imam Khomainy, Ekbatan and Fatemieh) in 2006. The samples were cultured on blood agar, and then the microbial agents were identified by differential and biochemical tests. Disinfectants that were used were as follow: Cidex (Glutaraldeyde), Sodium hypochlorite, Kereoeline 2.5%, Hygiene1%, Betadine, Alcohol 70%, Savlone 3.2%, and Chlorohexidine 1%. The efficacy of disinfectants was evaluated on 60 STAPHYLOCOCCUS EPIDERMIDIS and 60 PSEUDOMONAS AEROGINOSA through disk diffusion method. Data were gathered through a questionnaire and analysed using T-test and SPSS software. Contamination tests in the four hospitals were positive in 44.5% of cultures (178 cultures). The highest frequency of contamination was in Mobasher hospital with 38.2% and the less frequency was in Fatemieh hospital with 13.5%. From 400 samples, 52.2% of isolates were gram-positive bacteria and 47.7% were gram-negative bacteria. The most important bacteria isolated were as follow: E. coli, Staphylococcus epidemidis, Micrococci, Bacillus subtili and PSEUDOMONAS …