Authors
Joey Schyns, Qiang Bai, Cecilia Ruscitti, Coraline Radermecker, Sebastiaan De Schepper, Svetoslav Chakarov, Frédéric Farnir, Dimitri Pirottin, Florent Ginhoux, Guy Boeckxstaens, Fabrice Bureau, Thomas Marichal
Publication date
2019/9/3
Journal
Nature communications
Volume
10
Issue
1
Pages
3964
Publisher
Nature Publishing Group UK
Description
Resident tissue macrophages (RTM) can fulfill various tasks during development, homeostasis, inflammation and repair. In the lung, non-alveolar RTM, called interstitial macrophages (IM), importantly contribute to tissue homeostasis but remain little characterized. Here we show, using single-cell RNA-sequencing (scRNA-seq), two phenotypically distinct subpopulations of long-lived monocyte-derived IM, i.e. CD206+ and CD206−IM, as well as a discrete population of extravasating CD64+CD16.2+ monocytes. CD206+ IM are peribronchial self-maintaining RTM that constitutively produce high levels of chemokines and immunosuppressive cytokines. Conversely, CD206−IM preferentially populate the alveolar interstitium and exhibit features of antigen-presenting cells. In addition, our data support that CD64+CD16.2+ monocytes arise from intravascular Ly-6Clo patrolling monocytes that enter the tissue at steady-state …
Scholar articles
J Schyns, Q Bai, C Ruscitti, C Radermecker… - Nature communications, 2019