Authors
Nasar Khan, Muhammad Akmal, Muhammad Hayat, Muhammad Umar, Atta Ullah, Iqbal Ahmed, Kashif Rahim, Sadar Ali, Sulaiman Bahadar, Shamim Saleha
Publication date
2014/10
Journal
Hepatitis monthly
Volume
14
Issue
10
Publisher
Brieflands
Description
Background:
Distribution of Hepatitis C Virus (HCV) genotypes may be changed over time. Epidemiological Studies on distribution patterns of HCV genotypes in Pakistani population might assist for better treatment options and preventive strategies.
Objectives:
This study was conducted to determine distribution patterns of HCV genotypes in different geographical regions of Pakistan.
Patients and Methods:
In this cross-sectional study, 1818 randomly selected patients from different geographical regions of Pakistan, diagnosed with HCV infection by the third generation Enzyme Linked Immunosorbent Assay (ELISA), were included between April 2011 and December 2013. HCV RNA was detected in serum samples of patients by Reverse Transcription Polymerase Chain Reaction (RT-PCR) of the core region. Qualitative PCR was performed to determine viral load. HCV genotyping was performed by RT-nested PCR …
Scholar articles
N Khan, M Akmal, M Hayat, M Umar, A Ullah, I Ahmed… - Hepatitis monthly, 2014