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The aim of this work was to demonstrate the operation of a large pilot-scale submerged anaerobic membrane bioreactor (5.0 m³) for biogas production from municipal wastewater at ambient temperature of 25 °C. To the best of our knowledge, this is the largest one-stage submerged AnMBR that has ever been reported. This AnMBR realized a hydraulic retention time (HRT) of 6 h and a treatment capacity of 20 m³ d^-1, obtaining excellent effluent quality with COD removal efficiency over 90% and BOD₅ removal over 95%. The biogas yield of the AnMBR was 0.25–0.27 L g⁻¹ removed COD and 0.09–0.10 L L^-1 raw wastewater. The methane content of the biogas was at the range of 75%-81%. The COD and nitrogen mass balance were also identified based on long-term operation. The hollow-fiber membrane module realized a flux of 2.75–17.83 LMH. An online backwash chemical cleaning system helped to lower the …