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Cellulase produced from the marine fungus Cladosporium sphaerospermum through solid state fermentation (SSF) was investigated for its saccharification potential of seaweed biomass using the common green seaweed Ulva fasciata. The seaweed substrate, containing inoculated fungus with 60% moisture content, cultured at 25 °C and pH 4 for four days, showed optimum enzyme production. The enzyme, assayed for carboxymethyl cellulase and filter paper assay, showed an activity of 10.20 ± 0.40 U/g and 9.60 ± 0.64 U/g on a dry weight basis, respectively. Further, ionic liquid tolerance of the enzyme was studied in the presence of 1-ethyl-3-methylimidazolium acetate, 1-butyl-3-methylimidazolium chloride, 1-butyl-3-methylimidazolium trifluoromethanesulfonate and 1-butyl-1-methylpyrrolidinium trifluromethanesulfonate. At 10% v/v concentration, the enzyme retained 72.17 to 85.04% activity in all the ionic …