Authors
Christopher B Little, A Barai, D Burkhardt, SM Smith, AJ Fosang, Z Werb, M Shah, EW19950295 Thompson
Publication date
2009/12
Journal
Arthritis & Rheumatism: Official Journal of the American College of Rheumatology
Volume
60
Issue
12
Pages
3723-3733
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Description
Objective
To investigate the role of matrix metalloproteinase 13 (MMP‐13; collagenase 3) in osteoarthritis (OA).
Methods
OA was surgically induced in the knees of MMP‐13–knockout mice and wild‐type mice, and mice were compared. Histologic scoring of femoral and tibial cartilage aggrecan loss (0–3 scale), erosion (0–7 scale), and chondrocyte hypertrophy (0–1 scale), as well as osteophyte size (0–3 scale) and maturity (0–3 scale) was performed. Serial sections were stained for type X collagen and the MMP‐generated aggrecan neoepitope DIPEN.
Results
Following surgery, aggrecan loss and cartilage erosion were more severe in the tibia than femur (P < 0.01) and tibial cartilage erosion increased with time (P < 0.05) in wild‐type mice. Cartilaginous osteophytes were present at 4 weeks and underwent ossification, with size and maturity increasing by 8 weeks (P < 0.01). There was no difference between …
Total citations
Scholar articles
CB Little, A Barai, D Burkhardt, SM Smith, AJ Fosang… - Arthritis & Rheumatism: Official Journal of the American …, 2009