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The urban forest provides valuable ecosystem services for enhancing human well-being. Its structure and composition determine the quantity and quality of these services. There has been little research on the heterogeneity in structure and composition of urban forests in the Australasian region, especially in the centre of a highly dynamic and rapidly urbanizing city. This paper quantifies the structure and the composition of the urban forest of Melbourne, Australia's city centre. The effects of land tenure and land use on the heterogeneity of canopy cover, tree density and canopy size were explored. Species and family composition by land use, land ownership and street type were also analysed using the Shannon–Wiener and Jaccard similarity indices. Most of the canopy cover in the city centre is located on public land and is unevenly distributed across the municipality. The mean canopy cover (12.3%) is similar to …