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Bronchopulmonary dysplasia (BPD) is a lung disorder in newborns characterized by disrupted development of lung alveoli and microvasculature. This study performed multiomics comprising proteomics, lipidomics and metabolomics of BPD lung to delineate the pathophysiological response. The sample cohorts included BPD lungs (N= 9), healed lungs (N= 4), and control lungs matched for age (N= 10). MPLEx was employed to extract proteins, lipids, and metabolites from these samples. The extracted proteins were enzymatically broken down into peptides, labeled with Tandem Mass Tags (TMT), and subsequently analyzed through LCMS/MS. Comparative assessments of protein, lipid, and metabolite abundances were conducted across the diverse donor groups using ANOVA, and ontology enrichment analysis was carried out. The proteomic analysis identified 7,200 quantifiable …