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Bronchopulmonary Dysplasia (BPD) is a disease of prematurity related to the arrest of normal lung development. Whole lung proteomics was performed to identify individual proteins and pathways differentially regulated in BPD after the birth hospitalization. Pediatric human donors aged 1.3 to 3 years were classified based on history of prematurity and histopathologic severity of BPD (N= 3 “Recovered” BPD [rBPD] and N= 3 “Established” BPD [eBPD]) with respective full term (N= 6) age-matched controls. Lung tissues were biopsied (proteomics) and/or sectioned for imaging analyses. Proteins were identified by tandem mass spectroscopy requiring> 2 unique peptide fragments for confirmation and validated using Western Blot. Highly multiplexed (18 antibody panel) immunofluorescence (MxIF) microscopy was performed on lung sections to enumerate cell types and validate proteomics results …