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A better understanding of soil fertility factors that constraint positive crop response to fertilizer inputs will facilitate the improvement of soil nutrient management. In this study, a nutrient omission trial was carried out in a greenhouse condition to identify soil chemical properties limiting in non-responsive soils and to ascertain their effect on soybean (Glycine max) production. The treatments evaluated were control (distilled water), complete nutrient solution (all nutrients), and complete solution with the omission of each of N, P, K, Ca, Mg, and S and micronutrients (Fe, Zn, Cu, Mn, B, and Mo) arranged in a completely randomized design with three replications. After the greenhouse study, the identified limiting nutrients were tested with or without FertiSoil (commercial compost) in a 3 year field experiment. Results of the soil analyses showed low fertility status of the non-responsive soils. The sufficiency quotient index revealed non-responsive soils in all the sites to be predominantly limiting in P and K. The occurrence of other limiting nutrients was also identified: Pishegu (Zn, B), Serekpere (Mg, S), Daffiama Saapare (Ca, Mg, S), and Naaga (Mg, S, Zn, B). The nutrient inputs positively influenced soybean yield response in all the locations. The application of PKZnB with FertiSoil and FertiSoil alone significantly increased soybean grain yields by 585 and 477 kg ha⁻¹, respectively, at Pishegu. Soybean grain yields also increased by 585, 573, and 364 kg ha⁻¹ under the FertiSoil, PKMgS + FertiSoil, and PKMgS applications at Serekpere, respectively. At Daffiama Saapare, the highest (103%) percent increase in soybean grain yield was recorded from the …