Authors
Rehab M Abd El-Baky, Salwa M Masoud, Doaa S Mohamed, Nancy GFM Waly, Engy A Shafik, Dina A Mohareb, Azza Elkady, Mohamed M Elbadr, Helal F Hetta
Publication date
2020/2/3
Journal
Infection and Drug Resistance
Volume
13
Pages
323-332
Description
Background and Aim
The emergence of colistin-resistant strains is considered a great threat for patients with severe infections. Here, we investigate the prevalence and some possible mechanisms of colistin resistance among multidrug-resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa (P. aeruginosa).
Methods
Antimicrobial susceptibility was performed using disc diffusion methods while colistin resistance was detected by agar dilution method. Possible mechanisms for colistin resistance were studied by detection of mcr-1 and mcr-2 genes by conventional PCR, detection of efflux mechanisms using Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP), studying outer membrane protein profile and Lipopolysaccharide (LPS) profile of resistant isolates.
Results
It was found that MDR and XDR represented 96% and 87% of the isolated P. aeruginosa, respectively, and colistin resistance …
Scholar articles
RM Abd El-Baky, SM Masoud, DS Mohamed… - Infection and Drug Resistance, 2020