Authors
Helal F Hetta, Israa MS Al-Kadmy, Saba Saadoon Khazaal, Suhad Abbas, Ahmed Suhail, Mohamed A El-Mokhtar, Noura H Abd Ellah, Esraa A Ahmed, Rasha B Abd-Ellatief, Eman A El-Masry, Gaber El-Saber Batiha, Azza A Elkady, Nahed A Mohamed, Abdelazeem M Algammal
Publication date
2021/5/24
Journal
Scientific reports
Volume
11
Issue
1
Pages
10751
Publisher
Nature Publishing Group UK
Description
We aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression …
Total citations
Scholar articles
HF Hetta, IMS Al-Kadmy, SS Khazaal, S Abbas… - Scientific reports, 2021