Authors
Lizhen Li, Andrea Lundkvist, Daniel Andersson, Ulrika Wilhelmsson, Nobuo Nagai, Andrea C Pardo, Christina Nodin, Anders Ståhlberg, Karina Aprico, Kerstin Larsson, Takeshi Yabe, Lieve Moons, Andrew Fotheringham, Ioan Davies, Peter Carmeliet, Joan P Schwartz, Marcela Pekna, Mikael Kubista, Fredrik Blomstrand, Nicholas Maragakis, Michael Nilsson, Milos Pekny
Publication date
2008/3
Journal
Journal of Cerebral Blood Flow & Metabolism
Volume
28
Issue
3
Pages
468-481
Publisher
SAGE Publications
Description
Reactive astrocytes are thought to protect the penumbra during brain ischemia, but direct evidence has been lacking due to the absence of suitable experimental models. Previously, we generated mice deficient in two intermediate filament (IF) proteins, glial fibrillary acidic protein (GFAP) and vimentin, whose upregulation is the hallmark of reactive astrocytes. GFAP−/−Vim−/− mice exhibit attenuated posttraumatic reactive gliosis, improved integration of neural grafts, and posttraumatic regeneration. Seven days after middle cerebral artery (MCA) transection, infarct volume was 210 to 350% higher in GFAP−/−Vim−/− than in wild-type (WT) mice; GFAP−/−, Vim−/− and WT mice had the same infarct volume. Endothelin B receptor (ETBR) immunoreactivity was strong on cultured astrocytes and reactive astrocytes around infarct in WT mice but undetectable in GFAP−/−Vim−/− astrocytes. In WT astrocytes, ETBR colocalized …
Total citations
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Scholar articles
L Li, A Lundkvist, D Andersson, U Wilhelmsson… - Journal of Cerebral Blood Flow & Metabolism, 2008