Authors
Jim F Huggett, Carole A Foy, Vladimir Benes, Kerry Emslie, Jeremy A Garson, Ross Haynes, Jan Hellemans, Mikael Kubista, Reinhold D Mueller, Tania Nolan, Michael W Pfaffl, Gregory L Shipley, Jo Vandesompele, Carl T Wittwer, Stephen A Bustin
Publication date
2013/6/1
Journal
Clinical chemistry
Volume
59
Issue
6
Pages
892-902
Publisher
Oxford University Press
Description
There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acids, facilitating the measurement of small percentage differences and quantification of rare variants. dPCR may also be more reproducible and less susceptible to inhibition than quantitative real-time PCR (qPCR). Consequently, dPCR has the potential to have a substantial impact on research as well as diagnostic applications. However, as with qPCR, the ability to perform robust meaningful experiments requires careful design and adequate controls. To assist independent evaluation of experimental data, comprehensive disclosure of all relevant experimental details is required. To facilitate this process we present the Minimum Information for Publication of Quantitative Digital PCR Experiments guidelines. This report …
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