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Using pyrolysis technology to transform mulberry branches into wood vinegar with bacteriostatic properties can not only recycle the biomass wastes resources, but also bring considerable high added value. Four types of polyploidy mulberry branches were subjected to the slow pyrolysis process. Wood vinegar was collected in the operating temperature range of 25–510 °C. The yields of products were investigated, and the chemical characteristics of the refined wood vinegar as well as their antimicrobial performance were determined in detail. The results showed that the yield of the refined wood vinegar increased from 29% to 36% with the increase in chromosome number of the mulberry branches and meanwhile, the wood vinegar yield increased from 29% to 37% along with the growing year of the same diploid mulberry branches. All the refined wood vinegar exhibited promising antimicrobial activities. The most …