Authors
Antonia Puchner, Victoria Saferding, Michael Bonelli, Yohei Mikami, Melanie Hofmann, Julia S Brunner, Michael Caldera, Eliana Goncalves-Alves, Nikolaus B Binder, Anita Fischer, Elisabeth Simader, Carl-Walter Steiner, Harald Leiss, Silvia Hayer, Birgit Niederreiter, Thomas Karonitsch, Marije I Koenders, Bruno K Podesser, John J O’Shea, Jörg Menche, Josef S Smolen, Kurt Redlich, Stephan Blüml
Publication date
2018/10/1
Journal
Annals of the rheumatic diseases
Volume
77
Issue
10
Pages
1490-1497
Publisher
BMJ Publishing Group Ltd
Description
Objectives
Bone destruction in rheumatoid arthritis is mediated by osteoclasts (OC), which are derived from precursor cells of the myeloid lineage. The role of the two monocyte subsets, classical monocytes (expressing CD115, Ly6C and CCR2) and non-classical monocytes (which are CD115 positive, but low in Ly6C and CCR2), in serving as precursors for OC in arthritis is still elusive.
Methods
We investigated CCR2−/− mice, which lack circulating classical monocytes, crossed into hTNFtg mice for the extent of joint damage. We analysed monocyte subsets in hTNFtg and K/BxN serum transfer arthritis by flow cytometry. We sorted monocyte subsets and analysed their potential to differentiate into OC and their transcriptional response in response to RANKL by RNA sequencing. With these data, we performed a gene ontology enrichment analysis and gene set enrichment analysis.
Results
We show that in hTNFtg …
Scholar articles
A Puchner, V Saferding, M Bonelli, Y Mikami… - Annals of the rheumatic diseases, 2018