Authors
Jennifer Imm, Ehsan Pishva, Muhammadd Ali, Talitha L Kerrigan, Aaron Jeffries, Joe Burrage, Enrico Glaab, Emma L Cope, Kimberley M Jones, Nicholas D Allen, Katie Lunnon
Publication date
2021/7/1
Journal
Frontiers in Cell and Developmental Biology
Volume
9
Pages
647981
Publisher
Frontiers Media SA
Description
In development, differentiation from a pluripotent state results in global epigenetic changes, although the extent to which this occurs in induced pluripotent stem cell-based neuronal models has not been extensively characterized. In the present study, induced pluripotent stem cell colonies (33Qn1 line) were differentiated and collected at four time-points, with DNA methylation assessed using the Illumina Infinium Human Methylation EPIC BeadChip array. Dynamic changes in DNA methylation occurring during differentiation were investigated using a data-driven trajectory inference method. We identified a large number of Bonferroni-significant loci that showed progressive alterations in DNA methylation during neuronal differentiation. A gene–gene interaction network analysis identified 60 densely connected genes that were influential in the differentiation of neurons, with STAT3 being the gene with the highest connectivity.
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Scholar articles
J Imm, E Pishva, M Ali, TL Kerrigan, A Jeffries… - Frontiers in Cell and Developmental Biology, 2021