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Klebsiella pneumoniae is an important etiological agent of nosocomial infections. This study focuses on the antibacterial activity of Persea Americana extract against K. pneumoniae strains containing aminoglycoside genes. Broth microdilution technique was used to examine the antibacterial activity of P. americana extract on K. pneumoniae isolates. The phenolic components of its leaves were analysed by the HPLC-DAD system. The binding potential of the major phytochemical component of the extract, chlorogenic acid, to the KsgA was investigated by molecular docking. The stability of the resulting enzyme-ligand complexes was also assessed through molecular dynamics simulation. The result obtained showed that P. americana extract had antibacterial activity on aminoglycoside resistant strains. The binding potential of chlorogenic acid (main phenolic compound) to KsgA was found to be higher than the …