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Grammatical evolution (GE) is a form of grammar-based genetic programming. A particular feature of GE is that it adopts a distinction between the genotype and phenotype similar to that which exists in nature by using a grammar to map between the genotype and phenotype. Two variants of genotype representation are found in the literature, namely, binary and integer forms. For the first time we analyse and compare these two representations to determine if one has a performance advantage over the other. As such this study seeks to extend our understanding of GE by examining the impact of different genotypic representations in order to determine whether certain representations, and associated diversity-generation operators, improve GE’s efficiency and effectiveness. Four mutation operators using two different representations, binary and gray code representation, are investigated. The differing combinations of …