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The DnalK prote i n is a member of a highly conserved family of proteins (hsp 70) found in all living cells (3). The derived sequence of a 7lkD protein antigen from Mycobacteriumn tuberculosis shows it to be homologous to the E. coli DnaK protein (4). Sequence analysis of an open reading frame located 788 bp downstream from the dnaK gene of M1. tuberculosis indicates the occurrence of a 356 amino acid protein (fig. 1) sharing considerable sequence homology with the E. coli DnaJ protein (fig. 2) including conservation of fourtandem repeats of a motif consisting of Cys-XX-Cys-X-Gly-X-Gly (marked in fig. 1)(2). The DnaJ protein may resemble DnaK in being highly conserved amongst widely differing organisms.(it s) A a v ACAACAAAAACAGAAGCcCAGACGGGCTGACcGCCACATGCCCAAACcGAATGGCTCGAA 1429