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This chapter provides instructions for generating cDNA libraries with the h bacteriophage vectors gtl0 and gtll.~-8 Briefly, double-stranded cDNA containing EcoRI cohesive termini (this volume [38]) is ligated into the unique EcoRI cloning site present in hgtl0 or hgtl 1 DNA. Recombinant DNA is then packaged into viable phage particles which are plated on appropriate Escherichia coli hosts for amplification and screening. When only nucleic acid probes are available for library screening, hgtl0 is the vector of choice. When antibody probes are available for screening, hgtll is used since it is an expression vector (meaning that a fusion protein is formed between E. coli/3-galactosidase and eukaryotic protein from the cDNA inserts). Advantages of hgtl0 and gtll over the use of plasmid vector cDNA cloning include (1) the high efficiency of introduc-