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Currently major part of the world’s energy requirements is fulfilled by fossil resources. Severe economic and environmental problems along with their limited resources are still a major issue. Long-term environmental and commercial concern has focussed into huge research in the past decades to replace fossil fuels with renewable sources of energy. The main focus of energy research is lignocellulosic substrates, which seems to be the best for energy application due to easily availability, cost-effective and environment favourable production process. Therefore, lignocellulosic materials provide economical, environmental and energetic benefits with an alternative to traditional or high cost substrates. However, the close association of major constituents of lignocellulosic biomass forms different barriers, which hinders the enzymatic hydrolysis of cellulose and hemicellulose to different fermentable sugars. To overcome …