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Cassava based bioethanol production is a promising alternative to conventional fossil fuels and commercial production is already well established in several countries. A production based on small holder production may involve a transformation of the existing production system and it is therefore imperative to investigate opportunities and barriers for expansion of cassava production. This paper investigates the potential for an expansion of cassava cultivation for bioethanol production in Southern Mali. It is based on a questionnaire survey with 65 households in 2 villages in Loulouni municipality, which represent two major agro-ecological environments in the Soudan-zone in Mali. The results reveal that farmers are experienced cassava producers and are interested in an expansion of cassava cultivation for bioethanol production and that suitable areas are available, especially for an expansion of the Attieké …