Authors
Michael Fenech, Stefano Bonassi, Julie Turner, Cecilia Lando, Marcello Ceppi, Wushou Peter Chang, Nina Holland, Micheline Kirsch-Volders, Errol Zeiger, Maria Paola Bigatti, Claudia Bolognesi, Jia Cao, Giuseppe De Luca, Marina Di Giorgio, Lynnette R Ferguson, Aleksandra Fucic, Omar Garcia Lima, Valeria V Hadjidekova, Patrizia Hrelia, Alicja Jaworska, Gordana Joksic, AP Krishnaja, Tung-Kwang Lee, Antonietta Martelli, Michael J McKay, Lucia Migliore, Ekaterina Mirkova, Wolfgang-Ulrich Müller, Youichi Odagiri, Thierry Orsiere, Maria Rosaria Scarfı̀, Maria J Silva, Toshio Sofuni, Jordi Suralles, Giorgio Trenta, Irena Vorobtsova, Anne Vral, Andrea Zijno
Publication date
2003/1/10
Journal
Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Volume
534
Issue
1-2
Pages
45-64
Publisher
Elsevier
Description
One of the objectives of the HUman MicroNucleus (HUMN) project is to identify the methodological variables that have an important impact on micronucleus (MN) or micronucleated (MNed) cell frequencies measured in human lymphocytes using the cytokinesis-block micronucleus assay. In a previous study we had shown that the scoring criteria used were likely to be an important variable. To determine the extent of residual variation when laboratories scored cells from the same cultures using the same set of standard scoring criteria, an inter-laboratory slide-scoring exercise was performed among 34 laboratories from 21 countries with a total of 51 slide scorers involved. The results of this study show that even under these optimized conditions there is a great variation in the MN frequency or MNed cell frequency obtained by individual laboratories and scorers. All laboratories ranked correctly the MNed cell …
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Scholar articles
M Fenech, S Bonassi, J Turner, C Lando, M Ceppi… - Mutation Research/Genetic Toxicology and …, 2003
M Fenech, S Bonassi, J Turner, C Lando, M Ceppi… - Mutation Research-Genetic Toxicology and …, 2003