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This data set reports the (1) C and N isotopic composition of Sphagnum moss tissue collected from inside the SPRUCE experimental study plots from 2019-2021, as well as (2) rates of nitrogen fixation and methane oxidation measured for Sphagnum from 2017, 2019, and 2021. Both (1) and (2) also contain measurements of Sphagnum tissue % water content. This dataset contains two files in comma-separate (*.csv) format. Investigations leveraged the SPRUCE experiment where air and peat warming were combined in WEW treatments. SPRUCE is located at the 8.1-ha S1 Bog forest site in northern Minnesota, 40 km north of Grand Rapids, in the USDA Forest Service Marcell Experimental Forest (MEF). (1) Sphagnum C and N concentrations and 13C and 15N isotopic natural abundance were determined for three replicate Sphagnum tissue samples collected from inside the SPRUCE enclosures in July 2019-2021. Elemental and isotope analyses were conducted at the University of Georgia – Center for Applied Isotope Studies (CAIS https://cais.uga.edu/). Plant elemental analysis was performed by the micro-Dumas method, while isotopic natural abundance was measured by isotope ratio mass spectrometry. 13C natural abundance is expressed as the per mille (‰) deviation from the Pee Dee Belemnite standard (PDB) 13C:12C ratio (δ13C), while 15N natural abundance is expressed as the ‰ deviation from the N2 atmospheric 15N:14N ratio (δ15N). (2) Rates of nitrogen fixation and methane oxidation were measured using serum bottle incubations performed with Sphagnum collected inside the SPRUCE enclosures in July 2017, 2019, and …