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There is a gradual decline in good quality irrigation water for crop production. Farmers are forced to use poor quality water for crop production in peri-urban areas. Among the wastewater, sewage water generally contains higher amount of plant nutrients and dissolved organic matter. A case study was conducted to investigate the impact of untreated sewage water application on physicochemical properties and carbon sequestration in soil. For this purpose, peri-urban agricultural lands irrigated with sewage water from Patranala, Bhopal were selected. Such lands around both sides of the Patranala were being irrigated with sewage water for more than last 50 years. The soil samples were collected at various soil depths i.e., 0–15, 15–30, 30–45 and 45–60 cm from these fields; and sampling points were at about 2 km intervals along the channel direction and also at the distance interval of about 0.5, 1.0, 1.5 and 2.0 km …