Authors
Ricardo T Gazzinelli, Sara Hieny, Thomas A Wynn, Stanley Wolf, Alan Sher
Publication date
1993/7/1
Journal
Proceedings of the National Academy of Sciences
Volume
90
Issue
13
Pages
6115-6119
Description
Immunity against the intracellular protozoan Toxoplasma gondii is highly dependent on interferon gamma (IFN-gamma). We have previously shown that, in addition to T lymphocytes, natural killer (NK) cells can be stimulated by the parasite to produce this cytokine by a reaction requiring adherent accessory cells and tumor necrosis factor alpha. We now demonstrate that a recently characterized cytokine, interleukin 12 (IL-12), is also necessary for parasite-induced IFN-gamma synthesis by NK cells. Anti-IL-12 antibodies completely inhibited T. gondii or bacterial endotoxin-stimulated IFN-gamma production by NK-enriched spleen cells from severe combined immunodeficient mice. Moreover, potent NK cytokine responses were induced by the combination of IL-12 and tumor necrosis factor alpha. In addition, adherent spleen cells from scid/scid mice or thyoglycollate-elicited macrophages from BALB/c animals …
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Scholar articles
RT Gazzinelli, S Hieny, TA Wynn, S Wolf, A Sher - Proceedings of the National Academy of Sciences, 1993