Authors
Ricardo T Gazzinelli, Maria Wysocka, Sara Hieny, Tanya Scharton-Kersten, Allen Cheever, R Kühn, W Müller, Giorgio Trinchieri, Alan Sher
Publication date
1996/7/15
Journal
Journal of immunology (Baltimore, Md.: 1950)
Volume
157
Issue
2
Pages
798-805
Description
To examine the function of IL-10 synthesis during early infection with the intracellular protozoan Toxoplasma gondii, IL-10 knockout (KO) mice were inoculated with an avirulent parasite strain (ME-49). In contrast to control littermates that displayed 100% survival, the IL-10-deficient animals succumbed within the first 2 wk of the infection, with no evidence of enhanced parasite proliferation. The mortality in the IL-10 KO mice was associated with enhanced liver pathology characterized by increased cellular infiltration and intense necrosis. Levels of IL-12 and IFN-gamma in sera of infected IL-10-deficient animals were four- to sixfold higher than those in sera from control mice, as were mRNA levels for IFN-gamma, IL-1 beta, TNF-alpha, and IL-12 in lung tissue. Similarly, macrophages from IL-10 KO mice activated in vitro or in vivo with T. gondii produced higher levels of TNF-alpha and IL-12 than macrophages from …
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RT Gazzinelli, M Wysocka, S Hieny… - Journal of immunology (Baltimore, Md.: 1950), 1996