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2 This study investigates the willing-to-walk distance for measuring spatial accessibility of bus 3 stops and examines the effectiveness of alternative methods for generating mutually exclusive 4 transit service areas at the route level. We first investigate the walking distance to and from a 5 transit stop using on-board survey data. Two methods in Geographical Information Systems 6 (GIS), the Combination of Thiessen Polygon and Buffer (CTPB) and the Network Distance-7 based Service Area (NDSA), are compared as alternative strategies for generating mutually 8 exclusive service areas (MESAs). To examine the effectiveness of these two methods, all 9 MESAs are validated using a spider diagram generated from an on-board survey. Measures of 10 urban form are also statistically tested for comparison of the two different methods. A case study 11 of a single route, serving the Minneapolis-St. Paul metropolitan area …